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Mouse Dnmt3L Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Mouse Dnmt3L Antibody AP1062a
    Western blot analysis of Mouse Dnmt3L Antibody (Cat. #AP1062a) in Hela cell line lysates (35ug/lane). Dnmt3l (arrow) was detected using the purified Pab.
  • 1 - Mouse Dnmt3L Antibody AP1062a
    Dnmt3l Antibody (C403) (Cat. #AP1062a) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Dnmt3l antibody detected the Dnmt3l protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9CWR8
Other Accession Q1LZ50
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 47993 Da
Additional info
Gene ID 54427
Other Names DNA (cytosine-5)-methyltransferase 3-like, Dnmt3l
Target/Specificity This Mouse Dnmt3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-421 amino acids from mouse Dnmt3L.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Dnmt3L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Dnmt3l
Function Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases.
Cellular Location Nucleus.
Tissue Location Expressed in testis, thymus, ovary, and heart.
Research Areas

BACKGROUND

mDnmt3L (DNA methyltransferase 3L) does not appear to be catalytically active due to loss of the critical active site residues. It may function not directly as a DNA methyltransferase but rather as a regulator of methylation at imprinted loci. It is required specifically for the establishment of genomic imprints but is dispensable for their propagation. It is essential for the de novo methylation of single-copy DNA sequences. The protein is well-express in testis, thymus, ovary, and heart. mDnmt3L, which belongs to the 5-cytosine methyltransferase family, contains 1 ADD-type zinc finger.

REFERENCES

Okazaki, Y., et al., Nature 420(6915):563-573 (2002).
Bourc'his, D., et al., Science 294(5551):2536-2539 (2001).
Aapola, U., et al., Cytogenet. Cell Genet. 92 (1-2), 122-126 (2001).

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