|Application ||WB, E|
|Calculated MW||47993 Da|
|Other Names||DNA (cytosine-5)-methyltransferase 3-like, Dnmt3l|
|Target/Specificity||This Mouse Dnmt3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-421 amino acids from mouse Dnmt3L.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mouse Dnmt3L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases.|
|Tissue Location||Expressed in testis, thymus, ovary, and heart.|
mDnmt3L (DNA methyltransferase 3L) does not appear to be catalytically active due to loss of the critical active site residues. It may function not directly as a DNA methyltransferase but rather as a regulator of methylation at imprinted loci. It is required specifically for the establishment of genomic imprints but is dispensable for their propagation. It is essential for the de novo methylation of single-copy DNA sequences. The protein is well-express in testis, thymus, ovary, and heart. mDnmt3L, which belongs to the 5-cytosine methyltransferase family, contains 1 ADD-type zinc finger.
Okazaki, Y., et al., Nature 420(6915):563-573 (2002).
Bourc'his, D., et al., Science 294(5551):2536-2539 (2001).
Aapola, U., et al., Cytogenet. Cell Genet. 92 (1-2), 122-126 (2001).