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SPATC1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - SPATC1 Antibody (N-term) AP10622a
    All lanes : Anti-SPATC1 Antibody (N-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 62kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - SPATC1 Antibody (N-term) AP10622a
    SPATC1 Antibody (N-term) (Cat. #AP10622a) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the SPATC1 antibody detected the SPATC1 protein (arrow).
  • 14 - SPATC1 Antibody (N-term) AP10622a
    SPATC1 antibody (N-term) (Cat. #AP10622a) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPATC1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q76KD6
Other Accession Q148B6, NP_001127846.1, NP_940974.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 62399 Da
Additional info
Gene ID 375686
Other Names Speriolin, Spermatogenesis and centriole-associated protein 1, Spermatogenesis-associated protein 15, Spermatogenic cell-specific Cdc20-binding protein, SPATC1, SPATA15, SPRN
Target/Specificity This SPATC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SPATC1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPATC1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPATC1
Synonyms SPATA15, SPRN
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with the centrosomal pericentrin protein PCNT1 (By similarity). Located in the connecting piece of sperm. {ECO:0000250, ECO:0000269|PubMed:20542897}
Tissue Location Detected only in testis.
Research Areas
Alteration of sperm protein profile induced by cigarette smoking.
Author : Chen X1, Xu W2, Miao M3, Zhu Z2, Dai J2, Chen Z4, Fang P2, Wu J3, Nie D2, Wang L2, Wang Z2, Qiao Z5, Shi H3.
Acta Biochim Biophys Sin (Shanghai). 2015 Jun 10. pii: gmv045. [Epub ahead of print]
26063603

REFERENCES

Goto, M., et al. Hum. Reprod. 25(8):1884-1894(2010)
Goto, M., et al. J. Biol. Chem. 279(40):42128-42138(2004)

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