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FSTL1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - FSTL1 Antibody (C-term) AP10534b
    All lanes : Anti-FSTL1 Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: human placenta lysate Lane 3: human heart lysate Lane 4: mouse heart lysate Lane 5: rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - FSTL1 Antibody (C-term) AP10534b
    All lanes : Anti-FSTL1 Antibody (C-term) at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - FSTL1 Antibody (C-term) AP10534b
    FSTL1 Antibody (C-term) (Cat. #AP10534b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the FSTL1 antibody detected the FSTL1 protein (arrow).
  • 14 - FSTL1 Antibody (C-term) AP10534b
    FSTL1 antibody (C-term) (Cat. #AP10534b) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FSTL1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - FSTL1 Antibody (C-term) AP10534b
    FSTL1 Antibody (C-term) (Cat. #AP10534b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q12841
Other Accession Q9GKY0, NP_009016.1
Reactivity Human, Mouse, Rat
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 34986 Da
Additional info
Gene ID 11167
Other Names Follistatin-related protein 1, Follistatin-like protein 1, FSTL1, FRP
Target/Specificity This FSTL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-308 amino acids from the C-terminal region of human FSTL1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFSTL1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FSTL1
Synonyms FRP
Function May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin (By similarity).
Cellular Location Secreted.
Tissue Location Overexpressed in synovial tissues from rheumatoid arthritis.
Research Areas
A genetic polymorphism affects the risk and prognosis of renal cell carcinoma: association with follistatin-like protein 1 expression.
Author : Liu Y1, Han X2, Yu Y3, Ding Y1, Ni C1, Liu W1, Hou X1, Li Z1, Hou J4, Shen D2, Yin J1, Zhang H1, Thompson TC5, T
Sci Rep. 2016 May 26;6:26689. doi: 10.1038/srep26689.
27225192

REFERENCES

Kai, N., et al. Brain Res. 1073-1074, 60-70 (2006) :
Venter, J.C., et al. Science 291(5507):1304-1351(2001)

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