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NDUAB Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - NDUAB Antibody  (Center) AP10520c
    NDUAB Antibody (Center) (Cat. #AP10520c) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the NDUAB antibody detected the NDUAB protein (arrow).
  • 14 - NDUAB Antibody  (Center) AP10520c
    NDUAB antibody (Center) (Cat. #AP10520c) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUAB antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q86Y39
Other Accession NP_783313.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 14852 Da
Additional info
Gene ID 126328
Other Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11, Complex I-B147, CI-B147, NADH-ubiquinone oxidoreductase subunit B147, NDUFA11
Target/Specificity This NDUAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-92 amino acids from the Central region of human NDUAB.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUAB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFA11
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cellular Location Mitochondrion inner membrane; Multi-pass membrane protein; Matrix side
Research Areas

BACKGROUND

NDUFA11 encodes a subunit of the membrane-bound mitochondrial complex I. Complex I is composed of numerous subunits and functions as the NADH-ubiquinol reductase of the mitochondrial electron transport chain. Mutations in this gene are associated with severe mitochondrial complex I deficiency. Alternate splicing results in multiple transcript variants.

REFERENCES

Berger, I., et al. Ann. Neurol. 63(3):405-408(2008)
Takagaki, K., et al. Biochem. Biophys. Res. Commun. 309(2):351-358(2003)
Murray, J., et al. J. Biol. Chem. 278(16):13619-13622(2003)
Carroll, J., et al. J. Biol. Chem. 277(52):50311-50317(2002)
Carroll, J., et al. J. Biol. Chem. 277(52):50311-50317(2002)

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