注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   细胞生物学   >   CF150 Antibody (Center)   

CF150 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CF150 Antibody  (Center) AP10510c
    Western blot analysis of lysates from HT-29, THP-1 cell line, human cerebellum tissue lysate (from left to right), using CF150 Antibody (Center)(Cat. #AP10510c). AP10510c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • 4 - CF150 Antibody  (Center) AP10510c
    CF150 Antibody (Center) (Cat. #AP10510c) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 产品详情
  • 文献引用 : 2
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q8N884
Other Accession NP_612450.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 115004
Other Names Cyclic GMP-AMP synthase, cGAMP synthase, cGAS, h-cGAS, Mab-21 domain-containing protein 1, MB21D1, C6orf150
Target/Specificity This CF150 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human CF150.
Dilution WB~~1:500-1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCF150 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MB21D1
Synonyms C6orf150
Function Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double- stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.
Cellular Location Cytoplasm, cytosol
Tissue Location Expressed in the monocytic cell line THP1.
Research Areas
cGAS-STING Signaling Regulates Initial Innate Control of Cytomegalovirus Infection.
Author : Lio CJ1, McDonald B2, Takahashi M3, Dhanwani R3, Sharma N3, Huang J3, Pham E4, Benedict CA2, Sharma S5.
J Virol. 2016 Jun 22. pii: JVI.01040-16. [Epub ahead of print]
27334590
The DNA Sensor, Cyclic GMP-AMP Synthase, Is Essential for Induction of IFN-β during Chlamydia trachomatis Infection.
Author : Zhang Y1, Yeruva L2, Marinov A3, Prantner D4, Wyrick PB1, Lupashin V5, Nagarajan UM6.
J Immunol. 2014 Sep 1;193(5):2394-404. doi: 10.4049/jimmunol.1302718. Epub 2014 Jul 28.
25070851

BACKGROUND

The exact function of C6orf150 remains unknown.

REFERENCES

Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Mungall, A.J., et al. Nature 425(6960):805-811(2003)

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价