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WDR48 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - WDR48 Antibody (C-term) AP10505b
    Anti-WDR48_HUMAN at 1:1000 dilution + Rat testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 76 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - WDR48 Antibody (C-term) AP10505b
    WDR48 antibody (C-term) (Cat. #AP10505b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR48 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - WDR48 Antibody (C-term) AP10505b
    WDR48 Antibody (C-term) (Cat. #AP10505b) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q8TAF3
Other Accession Q4R2Z6, Q32PG3, NP_065890.1
Reactivity Rat
Predicted Bovine, Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 76210 Da
Additional info
Gene ID 57599
Other Names WD repeat-containing protein 48, USP1-associated factor 1, WD repeat endosomal protein, p80, WDR48, KIAA1449, UAF1
Target/Specificity This WDR48 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 603-630 amino acids from the C-terminal region of human WDR48.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWDR48 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WDR48
Synonyms KIAA1449, UAF1
Function Regulator of deubiquitinating complexes. Acts as a strong activator of USP1 and USP46 (PubMed:18082604, PubMed:19075014, PubMed:26388029). Enhances the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself (PubMed:18082604). Also activates deubiquitinating activity of complexes containing USP12 (PubMed:19075014, PubMed:27650958, PubMed:27373336). Docks at the distal end of the USP12 fingers domain and induces a cascade of structural changes leading to the activation of the enzyme (PubMed:27650958, PubMed:27373336). Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate (PubMed:19075014, PubMed:27373336). In complex with USP12, acts as a potential tumor suppressor by positively regulating PHLPP1 stability (PubMed:24145035). In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP (PubMed:12196293). In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1 (PubMed:18032488).
Cellular Location Nucleus. Cytoplasm. Lysosome. Late endosome. Note=Mainly in cytoplasmic compartments (PubMed:12196293, PubMed:18032488). In case of infection by papillomavirus HPV11, translocates to the nucleus via its interaction with papillomavirus HPV11 (PubMed:18032488)
Tissue Location Ubiquitous..
Research Areas

BACKGROUND

Regulator of deubiquitinating complexes. Acts as a strong activator of USP1 by enhancing the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself. Also activates deubiquitinating activity of complexes containing USP12 and USP46, respectively. Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP. In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1.

REFERENCES

Kee, Y., et al. J. Biol. Chem. 285(15):11252-11257(2010)
Cohn, M.A., et al. J. Biol. Chem. 284(8):5343-5351(2009)
Cote-Martin, A., et al. J. Virol. 82(3):1271-1283(2008)
Cohn, M.A., et al. Mol. Cell 28(5):786-797(2007)
Park, J., et al. J. Virol. 77(16):9041-9051(2003)

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