|Application ||WB, IHC-P, E|
|Calculated MW||93441 Da|
|Other Names||MICAL-like protein 1, Molecule interacting with Rab13, MIRab13, MICALL1, KIAA1668, MIRAB13|
|Target/Specificity||This MICALL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 752-781 amino acids from the C-terminal region of human MICALL1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MICALL1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Probable lipid-binding protein with higher affinity for phosphatidic acid, a lipid enriched in recycling endosome membranes. On endosome membranes, may act as a downstream effector of Rab proteins recruiting cytosolic proteins to regulate membrane tubulation. May be involved in a late step of receptor-mediated endocytosis regulating for instance endocytosed-EGF receptor trafficking. Alternatively, may regulate slow endocytic recycling of endocytosed proteins back to the plasma membrane. May indirectly play a role in neurite outgrowth.|
|Cellular Location||Recycling endosome membrane; Peripheral membrane protein. Late endosome membrane. Note=Localization to late endosomes is actin-dependent. Association to tubular recycling endosomes is regulated by RAB35 and ARF6|
Provided below are standard protocols that you may find useful for product applications.
MICALL1 may be a cytoskeletal regulator.
Sharma, M., et al. Mol. Biol. Cell 20(24):5181-5194(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Jin, J., et al. Curr. Biol. 14(16):1436-1450(2004)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)