注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   精选抗体   >   E2F2 Antibody (Center)   

E2F2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - E2F2 Antibody (Center) AP10481C
    All lanes : Anti-E2F2 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - E2F2 Antibody (Center) AP10481C
    E2F2 Antibody (Center) (Cat. #AP10481c) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the E2F2 antibody detected the E2F2 protein (arrow).
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q14209
Other Accession NP_004082.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 47506 Da
Additional info
Gene ID 1870
Other Names Transcription factor E2F2, E2F-2, E2F2
Target/Specificity This E2F2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the Central region of human E2F2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsE2F2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name E2F2
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.
Cellular Location Nucleus.
Tissue Location Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples
Research Areas

BACKGROUND

E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq].

REFERENCES

Revenko, A.S., et al. Mol. Cell. Biol. 30(22):5260-5272(2010)
Hayami, S., et al. Mol. Cancer 9, 59 (2010) :
Chen, J., et al. Cancer Causes Control 20(9):1769-1777(2009)
Cunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009)
Lal, A., et al. Mol. Cell 35(5):610-625(2009)

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价