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CTSA Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CTSA Antibody (N-term) AP10476a
    Anti-CTSA Antibody (N-term) at 1:2000 dilution + 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - CTSA Antibody (N-term) AP10476a
    Immunohistochemical analysis of paraffin-embedded H. kidney section using CTSA Antibody (N-term)(Cat#AP10476a). AP10476a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 1 - CTSA Antibody (N-term) AP10476a
    Western blot analysis of lysates from A431, Hela, HepG2, HT-1080 cell line and human placenta, kidney tissue lysate (from left to right), using CTSA Antibody (N-term)(Cat. #AP10476a). AP10476a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • 1 - CTSA Antibody (N-term) AP10476a
    CTSA Antibody (N-term) (Cat. #AP10476a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CTSA antibody detected the CTSA protein (arrow).
  • 1 - CTSA Antibody (N-term) AP10476a
    CTSA Antibody (N-term) (Cat. #AP10476a) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the CTSA antibody detected the CTSA protein (arrow).
  • 14 - CTSA Antibody (N-term) AP10476a
    CTSA antibody (N-term) (Cat. #AP10476a) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CTSA antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P10619
Other Accession NP_001161066.1, NP_000299.2, NP_001121167.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54466 Da
Additional info
Gene ID 5476
Other Names Lysosomal protective protein, Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, Protective protein cathepsin A, PPCA, Protective protein for beta-galactosidase, Lysosomal protective protein 32 kDa chain, Lysosomal protective protein 20 kDa chain, CTSA, PPGB
Target/Specificity This CTSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-45 amino acids from the N-terminal region of human CTSA.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCTSA Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTSA
Synonyms PPGB
Function Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.
Cellular Location Lysosome.
Research Areas
Chemical chaperone treatment for galactosialidosis: Effect of NOEV on β-galactosidase activities in fibroblasts.
Author : Hossain MA1, Higaki K2, Shinpo M1, Nanba E2, Suzuki Y3, Ozono K1, Sakai N4.
Brain Dev. 2015 Aug 7. pii: S0387-7604(15)00137-0. doi: 10.1016/j.braindev.2015.07.006. [Epub ahead of print]
26259553

BACKGROUND

CTSA encodes a glycoprotein which associates with lysosomal enzymes beta-galactosidase and neuraminidase to form a complex of high molecular weight multimers. The formation of this complex provides a protective role for stability and activity. Deficiencies in this gene are linked to multiple forms of galactosialidosis.

REFERENCES

Reich, M., et al. Immunol. Lett. 128(2):143-147(2010)
Bonten, E.J., et al. J. Biol. Chem. 284(41):28430-28441(2009)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Tatano, Y., et al. J. Med. Invest. 53 (1-2), 103-112 (2006) :
Lewandrowski, U., et al. Mol. Cell Proteomics 5(2):226-233(2006)

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