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CLIC6 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CLIC6 Antibody (Center) AP10474c
    CLIC6 Antibody (Center) (Cat. #AP10474c) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CLIC6 antibody detected the CLIC6 protein (arrow).
  • 14 - CLIC6 Antibody (Center) AP10474c
    CLIC6 antibody (Center) (Cat. #AP10474c) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLIC6 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - CLIC6 Antibody (Center) AP10474c
    CLIC6 Antibody (Center) (Cat. #AP10474c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q96NY7
Other Accession Q811Q2, Q8BHB9, NP_444507.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 73012 Da
Additional info
Gene ID 54102
Other Names Chloride intracellular channel protein 6, Parchorin, CLIC6, CLIC1L
Target/Specificity This CLIC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 539-565 amino acids from the Central region of human CLIC6.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCLIC6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CLIC6
Synonyms CLIC1L
Function May insert into membranes and form chloride ion channels. May play a critical role in water-secreting cells, possibly through the regulation of chloride ion transport (By similarity).
Cellular Location Cytoplasm. Cell membrane; Single-pass membrane protein Note=Predominantly cytoplasmic. Upon chloride ion efflux from the cell, it is translocated to the plasma membrane (By similarity)
Tissue Location Expressed in brain, placenta, pancreas and liver.
Research Areas

BACKGROUND

CLIC6 is a member of the chloride intracellular channel family of proteins. The gene is part of a large triplicated region found on chromosomes 1, 6, and 21. An alternatively spliced transcript variant has been described, but its biological validity has not been determined.

REFERENCES

Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009) :
Friedli, M., et al. Gene 320, 31-40 (2003) :
Strippoli, P., et al. Mamm. Genome 13(8):456-462(2002)
Scanlan, M.J., et al. Cancer Immun. 1, 4 (2001) :

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