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RABGAP1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RABGAP1 Antibody (N-term) AP10460a
    RABGAP1 Antibody (N-term) (Cat. #AP10460a) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RABGAP1 antibody detected the RABGAP1 protein (arrow).
  • 4 - RABGAP1 Antibody (N-term) AP10460a
    RABGAP1 Antibody (N-term) (Cat. #AP10460a) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession Q9Y3P9
Other Accession A2AWA9, NP_036329.3
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 121737 Da
Additional info
Gene ID 23637
Other Names Rab GTPase-activating protein 1, GAP and centrosome-associated protein, Rab6 GTPase-activating protein GAPCenA, RABGAP1
Target/Specificity This RABGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human RABGAP1.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRABGAP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RABGAP1
Function May act as a GTPase-activating protein of RAB6A. May play a role in microtubule nucleation by centrosome. May participate in a RAB6A-mediated pathway involved in the metaphase- anaphase transition.
Cellular Location Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Predominantly cytosolic but also associated with the centrosome
Research Areas

BACKGROUND

RABGAP1 may act as a GTPase-activating protein of RAB6A and play a role in microtubule nucleation by centrosome. It may participate in a RAB6A-mediated pathway involved in the metaphase-anaphase transition.

REFERENCES

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Ishibashi, K., et al. Genes Cells 14(1):41-52(2009)
Echard, A., et al. Mol. Biol. Cell 11(11):3819-3833(2000)
Opdam, F.J., et al. J. Cell. Sci. 113 (PT 15), 2725-2735 (2000) :
Cuif, M.H., et al. EMBO J. 18(7):1772-1782(1999)

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