注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   精选抗体   >   ENSA Antibody (Center)   

ENSA Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ENSA Antibody (Center) AP10456c
    ENSA Antibody (Center) (Cat. #AP10456c) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ENSA antibody detected the ENSA protein (arrow).
  • 1 - ENSA Antibody (Center) AP10456c
    ENSA Antibody (Center) (Cat. #AP10456c) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the ENSA antibody detected the ENSA protein (arrow).
  • 4 - ENSA Antibody (Center) AP10456c
    ENSA Antibody (Center) (Cat. #AP10456c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession O43768
Other Accession Q7ZXH9, P60841, P68211, P60840, Q1L8X2, Q5ZIF8, P68210, NP_996927.1, NP_996929.1
Reactivity Human, Mouse
Predicted Bovine, Chicken, Zebrafish, Pig, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 13389 Da
Additional info
Gene ID 2029
Other Names Alpha-endosulfine, ARPP-19e, ENSA
Target/Specificity This ENSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the Central region of human ENSA.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsENSA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ENSA
Function Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.
Cellular Location Cytoplasm.
Tissue Location Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas
Research Areas

BACKGROUND

ENSA belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes.

REFERENCES

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Gabrielsson, B.G., et al. Mol. Cell. Biochem. 258 (1-2), 65-71 (2004) :
Thameem, F., et al. Mol. Genet. Metab. 81(1):16-21(2004)

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价