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>   首页   >   产品   >   一抗   >   癌症   >   ANGPT1 Antibody (C-term)   

ANGPT1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ANGPT1 Antibody (C-term) AP10451b
    Anti-ANGPT1 Antibody (C-term)at 1:2000 dilution + mouse lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ANGPT1 Antibody (C-term) AP10451b
    ANGPT1 Antibody (C-term) (Cat. #AP10451b) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the ANGPT1 antibody detected the ANGPT1 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q15389
Other Accession Q9BDY8, O08538, O18920, NP_001137.2
Reactivity Human, Mouse
Predicted Bovine, Mouse, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57513 Da
Additional info
Gene ID 284
Other Names Angiopoietin-1, ANG-1, ANGPT1, KIAA0003
Target/Specificity This ANGPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-402 amino acids from the C-terminal region of human ANGPT1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsANGPT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ANGPT1
Synonyms KIAA0003
Function Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.
Cellular Location Secreted.
Research Areas

BACKGROUND

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

REFERENCES

Fang, X., et al. J. Biol. Chem. 285(34):26211-26222(2010)
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Choe, J.Y., et al. Joint Bone Spine 77(4):340-344(2010)
Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010)
Chen, J., et al. Hum. Mol. Genet. 19(12):2524-2533(2010)

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