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SMYD2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - SMYD2 Antibody (N-term) AP1044a
    All lanes : Anti-SMYD2 Antibody (N-term) at 1:2000 dilution Lane 1: HT-1080 whole cell lysates Lane 2: mouse heart lysates Lane 3: Hela whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - SMYD2 Antibody (N-term) AP1044a
    Western blot analysis of SYMD2 (arrow) using rabbit polyclonal SYMD2 Antibody (N-term) (Cat.#AP1044a).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SYMD2 gene (Lane 2) (Origene Technologies).
  • 1 - SMYD2 Antibody (N-term) AP1044a
    SMYD2 Antibody (N-term) (Cat.#AP1044a) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SMYD2 antibody detected the SMYD2 protein (arrow).
  • 1 - SMYD2 Antibody (N-term) AP1044a
    SMYD2 Antibody (N-term) (Cat.#AP1044a) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the SMYD2 antibody detected the SMYD2 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9NRG4
Other Accession Q7M6Z3, Q8R5A0
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49688 Da
Additional info
Gene ID 56950
Other Names N-lysine methyltransferase SMYD2, 211-, HSKM-B, Histone methyltransferase SMYD2, Lysine N-methyltransferase 3C, SET and MYND domain-containing protein 2, SMYD2, KMT3C
Target/Specificity This SMYD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-152 amino acids from the N-terminal region of human SMYD2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMYD2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMYD2
Synonyms KMT3C
Function Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.
Cellular Location Cytoplasm, cytosol. Nucleus
Research Areas

BACKGROUND

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM].

REFERENCES

Abu-Farha,M.,Mol. Cell Proteomics 7 (3), 560-572 (2008)
Ewing,R.M.,Mol. Syst. Biol. 3, 89 (2007)

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