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AIF1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - AIF1 Antibody (N-term) AP10449a
    All lanes : Anti-AIF1 Antibody (N-term) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - AIF1 Antibody (N-term) AP10449a
    All lanes : Anti-AIF1 Antibody (N-term) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: THP-1 whole cell lysate Lane 4: human spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - AIF1 Antibody (N-term) AP10449a
    AIF1 antibody (N-term) (Cat. #AP10449a) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AIF1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P55008
Other Accession NP_001614.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 16703 Da
Additional info
Gene ID 199
Other Names Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, AIF1, G1, IBA1
Target/Specificity This AIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-36 amino acids from the N-terminal region of human AIF1.
Dilution WB~~1:2000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAIF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AIF1
Synonyms G1, IBA1
Function Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Cellular Location Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side Note=Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis
Tissue Location Detected in T-lymphocytes and peripheral blood mononuclear cells.
Research Areas
Leukotoxin (Leukothera®) targets active leukocyte function antigen-1 (LFA-1) protein and triggers a lysosomal mediated cell death pathway.
Author : DiFranco KM1, Gupta A, Galusha LE, Perez J, Nguyen TV, Fineza CD, Kachlany SC.
J Biol Chem. 2012 May 18;287(21):17618-27. doi: 10.1074/jbc.M111.314674. Epub 2012 Mar 30.
22467872

BACKGROUND

AIF1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.

REFERENCES

Clancy, R.M., et al. Arthritis Rheum. 62(11):3415-3424(2010)
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Jia, J., et al. Pediatr. Res. 67(1):29-34(2010)
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :

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