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AP1S3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - AP1S3 Antibody (N-term) AP10390a
    AP1S3 Antibody (N-term) (Cat. #AP10390a) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the AP1S3 antibody detected the AP1S3 protein (arrow).
  • 14 - AP1S3 Antibody (N-term) AP10390a
    AP1S3 antibody (N-term) (Cat. #AP10390a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AP1S3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q96PC3
Other Accession NP_001034658.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 130340
Other Names AP-1 complex subunit sigma-3, Adaptor protein complex AP-1 subunit sigma-1C, Adaptor-related protein complex 1 subunit sigma-1C, Clathrin assembly protein complex 1 sigma-1C small chain, Golgi adaptor HA1/AP1 adaptin sigma-1C subunit, Sigma 1C subunit of AP-1 clathrin, Sigma-adaptin 1C, Sigma1C-adaptin, AP1S3
Target/Specificity This AP1S3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-37 amino acids from the N-terminal region of human AP1S3.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAP1S3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AP1S3
Function Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Cellular Location Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex
Tissue Location Widely expressed.
Research Areas

REFERENCES

Rose, J. Phd, et al. Mol. Med. (2010) In press : Roeth, J.F., et al. J. Cell Biol. 167(5):903-913(2004) Hirst, J., et al. Mol. Biol. Cell 12(11):3573-3588(2001) Boehm, M., et al. Mol. Biol. Cell 12(10):2907-2920(2001) Kirchhausen, T. Nat. Rev. Mol. Cell Biol. 1(3):187-198(2000)

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