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EFTUD1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - EFTUD1 Antibody (C-term) AP10373b
    EFTUD1 Antibody (C-term) (Cat. #AP10373b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EFTUD1 antibody detected the EFTUD1 protein (arrow).
  • 1 - EFTUD1 Antibody (C-term) AP10373b
    EFTUD1 Antibody (C-term) (Cat. #AP10373b) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the EFTUD1 antibody detected the EFTUD1 protein (arrow).
  • 14 - EFTUD1 Antibody (C-term) AP10373b
    EFTUD1 antibody (C-term) (Cat. #AP10373b) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EFTUD1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - EFTUD1 Antibody (C-term) AP10373b
    EFTUD1 Antibody (C-term) (Cat. #AP10373b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q7Z2Z2
Other Accession Q8C0D5, NP_078856.4, NP_001035700.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 125430 Da
Additional info
Gene ID 79631
Other Names Elongation factor Tu GTP-binding domain-containing protein 1, Elongation factor-like 1, Protein FAM42A, EFTUD1, EFL1, FAM42A
Target/Specificity This EFTUD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1062-1090 amino acids from the C-terminal region of human EFTUD1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEFTUD1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EFTUD1
Synonyms EFL1, FAM42A
Function Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits.
Research Areas

REFERENCES

Nicolas, E., et al. Eur. J. Hum. Genet. 18(10):1107-1113(2010)
Willer, C.J., et al. Nat. Genet. 41(1):25-34(2009)
Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001)

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