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CEPT1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 59 - CEPT1 Antibody (N-term) AP10372a
    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using AP10372a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 59 - CEPT1 Antibody (N-term) AP10372a
    Immunohistochemical analysis of paraffin-embedded Human breast tissue using AP10372a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - CEPT1 Antibody (N-term) AP10372a
    All lanes : Anti-CEPT1 Antibody (N-term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: Human cerebellum tissue lysate Lane 3: SK-BR-3 whole cell lysate Lane 4: Human placenta tissue lysate Lane 5: Mouse spleen tissue lysate Lane 6: Mouse brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CEPT1 Antibody (N-term) AP10372a
    All lanes : Anti-CEPT1 Antibody (N-term) at 1:4000 dilution Lane 1: HL-60 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Human cerebellum tissue lysate Lane 4: SK-BR-3 whole cell lysate Lane 5: Mouse brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - CEPT1 Antibody (N-term) AP10372a
    Anti-CEPT1 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, E
Primary Accession Q9Y6K0
Other Accession Q7ZYQ3, Q6AXM5, Q8BGS7, NP_001007795.1, NP_006081.1
Reactivity Human, Mouse
Predicted Mouse, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46554 Da
Additional info
Gene ID 10390
Other Names Choline/ethanolaminephosphotransferase 1, hCEPT1, CEPT1
Target/Specificity This CEPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of human CEPT1.
Dilution IHC-P-Leica~~1:500
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCEPT1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CEPT1
Function Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis from CDP-choline and CDP- ethanolamine, respectively. Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus membrane; Multi-pass membrane protein
Tissue Location Ubiquitously expressed.
Research Areas

BACKGROUND

Cholinephosphotransferase catalyses the final step in the synthesis of phosphatidylcholine by the transfer of phosphocholine from CDP-choline to diacylglycerol. The synthesis of phosphatidylethanolamine by ethanolaminephosphotransferase occurs using an analogous reaction. This gene codes for a choline/ethanolaminephosphotransferase. The protein can synthesize either choline- or ethanolamine- containing phospholipids. Two alternatively spliced transcripts encoding the same isoform have been identified.

REFERENCES

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Wright, M.M., et al. Lipids 37(7):663-672(2002)
Henneberry, A.L., et al. Biochem. J. 339 (PT 2), 291-298 (1999) :

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