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Dnmt3b Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Dnmt3b Antibody AP1035A
    Anti-Dnmt3b Antibody (C403)at 1:2000 dilution + T47D whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 95 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Dnmt3b Antibody AP1035A
    All lanes : Anti-Dnmt3b Antibody (C403) at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 95 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - Dnmt3b Antibody AP1035A
    Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody (Cat.#AP1035a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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  • 文献引用 : 14
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession Q9UBC3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 1789
Other Names DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, MHsaIIIB, DNMT3B
Target/Specificity This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-417 amino acids from human Dnmt3b.
Dilution WB~~1:2000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDnmt3b Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNMT3B
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co- repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells (PubMed:27153398).
Cellular Location Nucleus
Tissue Location Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate
Research Areas
5-Aza-2'-deoxycytidine induces human Tenon's capsule fibroblasts differentiation and fibrosis by up-regulating TGF-β type I receptor.
Author : Fu S1,Sun L1,Zhang X1,Shi H1,Xu K1,Xiao Y2,Ye W3.
Exp Eye Res. 2017 Sep 9. pii: S0014-4835(17)30104-5. doi: 10.1016/j.exer.2017.09.004. [Epub ahead of print]
28893564
Fractionated low-dose exposure to ionizing radiation leads to DNA damage, epigenetic dysregulation, and behavioral impairment.
Author : Koturbash I1,Jadavji NM2,Kutanzi K1,Rodriguez-Juarez R1,Kogosov D1,Metz GAS2,3,Kovalchuk O1,3.
Environ Epigenet. 2017 Jan 31;2(4):dvw025. doi: 10.1093/eep/dvw025. eCollection 2016 Dec.
29492301
T cell receptor (TCR) and transforming growth factor β (TGF-β) signaling converge on DNA (cytosine-5)-methyltransferase to control forkhead box protein 3 (foxp3) locus methylation and inducible regulatory T cell differentiation.
Author : Li C1, Ebert PJ, Li QJ.
J Biol Chem. 2013 Jun 28;288(26):19127-39. doi: 10.1074/jbc.M113.453357. Epub 2013 May 16.
23687305
OxLDL up-regulates microRNA-29b, leading to epigenetic modifications of MMP-2/MMP-9 genes: a novel mechanism for cardiovascular diseases.
Author : Chen KC1, Wang YS, Hu CY, Chang WC, Liao YC, Dai CY, Juo SH.
FASEB J. 2011 May;25(5):1718-28. doi: 10.1096/fj.10-174904. Epub 2011 Jan 25.
21266537
Functional switching of TGF-beta1 signaling in liver cancer via epigenetic modulation of a single CpG site in TTP promoter.
Author : Sohn BH, Park IY, Lee JJ, Yang SJ, Jang YJ, Park KC, Kim DJ, Lee DC, Sohn HA, Kim TW, Yoo HS, Choi JY, Bae YS, Yeom YI.
Gastroenterology. 2010 May;138(5):1898-908. doi: 10.1053/j.gastro.2009.12.044. Epub 2009 Dec 29.
20038433
Systems-level dynamic analyses of fate change in murine embryonic stem cells.
Author : Lu R, Markowetz F, Unwin RD, Leek JT, Airoldi EM, MacArthur BD, Lachmann A, Rozov R, Ma'ayan A, Boyer LA, Troyanskaya OG, Whetton AD, Lemischka IR.
Nature. 2009 Nov 19;462(7271):358-62. doi: 10.1038/nature08575.
19924215
DNA methyltransferase expression in the human endometrium: down-regulation by progesterone and estrogen.
Author : Yamagata Y, Asada H, Tamura I, Lee L, Maekawa R, Taniguchi K, Taketani T, Matsuoka A, Tamura H, Sugino N.
Hum Reprod. 2009 May;24(5):1126-32. doi: 10.1093/humrep/dep015. Epub 2009 Feb 6.
19202141
MicroRNA-29 family reverts aberrant methylation in lung cancer by targeting DNA methyltransferases 3A and 3B.
Author : Fabbri M, Garzon R, Cimmino A, Liu Z, Zanesi N, Callegari E, Liu S, Alder H, Costinean S, Fernandez-Cymering C, Volinia S, Guler G, Morrison CD, Chan KK, Marcucci G, Calin GA, Huebner K, Croce CM.
Proc Natl Acad Sci U S A. 2007 Oct 2;104(40):15805-10. Epub 2007 Sep 21.
17890317
Role of epigenetic effectors in maintenance of the long-term persistent bystander effect in spleen in vivo.
Author : Koturbash I, Boyko A, Rodriguez-Juarez R, McDonald RJ, Tryndyak VP, Kovalchuk I, Pogribny IP, Kovalchuk O.
Carcinogenesis. 2007 Aug;28(8):1831-8. Epub 2007 Mar 7.
17347136
Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein.
Author : Park IY, Sohn BH, Yu E, Suh DJ, Chung YH, Lee JH, Surzycki SJ, Lee YI.
Gastroenterology. 2007 Apr;132(4):1476-94. Epub 2007 Jan 25.
17408664
Effect of long-term tamoxifen exposure on genotoxic and epigenetic changes in rat liver: implications for tamoxifen-induced hepatocarcinogenesis.
Author : Tryndyak VP, Muskhelishvili L, Kovalchuk O, Rodriguez-Juarez R, Montgomery B, Churchwell MI, Ross SA, Beland FA, Pogribny IP.
Carcinogenesis. 2006 Aug;27(8):1713-20. Epub 2006 Apr 22.
16632870
Irradiation induces DNA damage and modulates epigenetic effectors in distant bystander tissue in vivo.
Author : Koturbash I, Rugo RE, Hendricks CA, Loree J, Thibault B, Kutanzi K, Pogribny I, Yanch JC, Engelward BP, Kovalchuk O.
Oncogene. 2006 Jul 20;25(31):4267-75. Epub 2006 Mar 13.
16532033
Fractionated low-dose radiation exposure leads to accumulation of DNA damage and profound alterations in DNA and histone methylation in the murine thymus.
Author : Pogribny I, Koturbash I, Tryndyak V, Hudson D, Stevenson SM, Sedelnikova O, Bonner W, Kovalchuk O.
Mol Cancer Res. 2005 Oct;3(10):553-61.
16254189
Epigenetic reactivation of tumor suppressor genes by a novel small-molecule inhibitor of human DNA methyltransferases.
Author : Brueckner B, Garcia Boy R, Siedlecki P, Musch T, Kliem HC, Zielenkiewicz P, Suhai S, Wiessler M, Lyko F.
Cancer Res. 2005 Jul 15;65(14):6305-11.
16024632

BACKGROUND

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3b is a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF)syndrome.

REFERENCES

Okano, M., et al., Cell 99(3):247-257 (1999). Yin, B., et al., Zhongguo Yi Xue Ke Xue Yuan Xue Bao 21(6):431-438 (1999). Okano, M., et al., Nat. Genet. 19(3):219-220 (1998).

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