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Dnmt3a Antibody (Center D472)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Dnmt3a Antibody (Center D472) AP1034c
    Western blot analysis of anti-DNMT3A Antibody (Center D472) (Cat.#AP1034c) in Jurkat cell line lysates (35ug/lane).DNMT3A(arrow) was detected using the purified Pab.
  • 14 - Dnmt3a Antibody (Center D472) AP1034c
    Formalin-fixed and paraffin-embedded human kidney tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9Y6K1
Other Accession Q1LZ53, O88508, Q4W5Z4
Reactivity Human
Predicted Mouse, Rat, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 1788
Other Names DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A
Target/Specificity This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from the Central region of human Dnmt3a.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDnmt3a Antibody (Center D472) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNMT3A
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
Cellular Location Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin
Tissue Location Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
Research Areas
DNMT3a expression pattern and its prognostic value in lung adenocarcinoma.
Author : Husni RE1,Shiba-Ishii A2,Iiyama S1,Shiozawa T1,Kim Y1,Nakagawa T1,Sato T1,Kano J2,Minami Y3,Noguchi M4.
Lung Cancer. 2016 Jul;97:59-65. doi: 10.1016/j.lungcan.2016.04.018. Epub 2016 Apr 27.
27237029
Epigenetic regulation of motor neuron cell death through DNA methylation.
Author : Chestnut BA, Chang Q, Price A, Lesuisse C, Wong M, Martin LJ.
J Neurosci. 2011 Nov 16;31(46):16619-36. doi: 10.1523/JNEUROSCI.1639-11.2011.
22090490

BACKGROUND

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

REFERENCES

Xie, S., et al., Gene 236(1):87-95 (1999). Robertson, K.D., et al., Nucleic Acids Res. 27(11):2291-2298 (1999).

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