注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   精选抗体   >   Dnmt3a Antibody   

Dnmt3a Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
标注"皇冠"的产品,是满足高质量标准的产品。
了解更多...
  • 1 - Dnmt3a Antibody AP1034a
    All lanes : Anti-Dnmt3a Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: T47D whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: Rat heart tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 102 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Dnmt3a Antibody AP1034a
    All lanes : Anti-Dnmt3a Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: Human skeletal muscle tissue lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat heart tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 102 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 文献引用 : 10
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9Y6K1
Other Accession Q1LZ53, O88508, Q4W5Z4
Reactivity Human, Mouse, Rat
Predicted Mouse, Rat, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 1788
Other Names DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A
Target/Specificity This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from human Dnmt3a.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDnmt3a Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNMT3A
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
Cellular Location Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin
Tissue Location Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
Research Areas
Glucocorticoid-induced S-adenosylmethionine enhances the interferon signaling pathway by restoring STAT1 protein methylation in hepatitis B virus-infected cells.
Author : Bing Y1, Zhu S1, Yu G1, Li T1, Liu W1, Li C1, Wang Y1, Qi H1, Guo T1, Yuan Y1, He Y1, Liu Z2, Liu Q3.
J Biol Chem. 2014 Nov 21;289(47):32639-55. doi: 10.1074/jbc.M114.589689. Epub 2014 Sep 30.
25271158
Hiwi mediated tumorigenesis is associated with DNA hypermethylation.
Author : Siddiqi S, Terry M, Matushansky I.
PLoS One. 2012;7(3):e33711. doi: 10.1371/journal.pone.0033711. Epub 2012 Mar 16.
22438986
Sex-specific radiation-induced microRNAome responses in the hippocampus, cerebellum and frontal cortex in a mouse model.
Author : Koturbash I, Zemp F, Kolb B, Kovalchuk O.
Mutat Res. 2011 Jun 17;722(2):114-8. doi: 10.1016/j.mrgentox.2010.05.007. Epub 2010 May 15.
20478395
OxLDL up-regulates microRNA-29b, leading to epigenetic modifications of MMP-2/MMP-9 genes: a novel mechanism for cardiovascular diseases.
Author : Chen KC1, Wang YS, Hu CY, Chang WC, Liao YC, Dai CY, Juo SH.
FASEB J. 2011 May;25(5):1718-28. doi: 10.1096/fj.10-174904. Epub 2011 Jan 25.
21266537
DNA methyltransferase expression in the human endometrium: down-regulation by progesterone and estrogen.
Author : Yamagata Y, Asada H, Tamura I, Lee L, Maekawa R, Taniguchi K, Taketani T, Matsuoka A, Tamura H, Sugino N.
Hum Reprod. 2009 May;24(5):1126-32. doi: 10.1093/humrep/dep015. Epub 2009 Feb 6.
19202141
Role of epigenetic effectors in maintenance of the long-term persistent bystander effect in spleen in vivo.
Author : Koturbash I, Boyko A, Rodriguez-Juarez R, McDonald RJ, Tryndyak VP, Kovalchuk I, Pogribny IP, Kovalchuk O.
Carcinogenesis. 2007 Aug;28(8):1831-8. Epub 2007 Mar 7.
17347136
Up-regulation of DNA-methyltransferase 3A expression is associated with hypomethylation of intron 25 in human testicular germ cell tumors.
Author : Ishii T, Kohu K, Yamada S, Ishidoya S, Kanto S, Fuji H, Moriya T, Satake M, Arai Y.
Tohoku J Exp Med. 2007 Jun;212(2):177-90.
17548962
Effect of long-term tamoxifen exposure on genotoxic and epigenetic changes in rat liver: implications for tamoxifen-induced hepatocarcinogenesis.
Author : Tryndyak VP, Muskhelishvili L, Kovalchuk O, Rodriguez-Juarez R, Montgomery B, Churchwell MI, Ross SA, Beland FA, Pogribny IP.
Carcinogenesis. 2006 Aug;27(8):1713-20. Epub 2006 Apr 22.
16632870
Irradiation induces DNA damage and modulates epigenetic effectors in distant bystander tissue in vivo.
Author : Koturbash I, Rugo RE, Hendricks CA, Loree J, Thibault B, Kutanzi K, Pogribny I, Yanch JC, Engelward BP, Kovalchuk O.
Oncogene. 2006 Jul 20;25(31):4267-75. Epub 2006 Mar 13.
16532033
Fractionated low-dose radiation exposure leads to accumulation of DNA damage and profound alterations in DNA and histone methylation in the murine thymus.
Author : Pogribny I, Koturbash I, Tryndyak V, Hudson D, Stevenson SM, Sedelnikova O, Bonner W, Kovalchuk O.
Mol Cancer Res. 2005 Oct;3(10):553-61.
16254189

BACKGROUND

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

REFERENCES

Xie, S., et al., Gene 236(1):87-95 (1999). Robertson, K.D., et al., Nucleic Acids Res. 27(11):2291-2298 (1999).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价