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Dnmt1 Antibody (C-term S1602)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - Dnmt1 Antibody (C-term S1602) AP1032A
    Dnmt1 Antibody (C-term S1602) (Cat. #AP1032a) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 1 - Dnmt1 Antibody (C-term S1602) AP1032A
    The anti-Dnmt1 C-term Pab (Cat. #AP1032a) is used in Western blot to detect Dnmt1 in Jurkat cell lysate.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession P26358
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 183165 Da
Additional info
Gene ID 1786
Other Names DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, MHsaI, MCMT, DNMT1, AIM, CXXC9, DNMT
Target/Specificity This Dnmt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1588-1616 amino acids from the C-terminal region of human Dnmt1.
Dilution FC~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDnmt1 Antibody (C-term S1602) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNMT1
Synonyms AIM, CXXC9, DNMT
Function Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306).
Cellular Location Nucleus.
Tissue Location Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Research Areas
Glucocorticoid-induced S-adenosylmethionine enhances the interferon signaling pathway by restoring STAT1 protein methylation in hepatitis B virus-infected cells.
Author : Bing Y1, Zhu S1, Yu G1, Li T1, Liu W1, Li C1, Wang Y1, Qi H1, Guo T1, Yuan Y1, He Y1, Liu Z2, Liu Q3.
J Biol Chem. 2014 Nov 21;289(47):32639-55. doi: 10.1074/jbc.M114.589689. Epub 2014 Sep 30.
25271158
Epigenetic mechanisms of age-dependent KIR2DL4 expression in T cells.
Author : Li G, Weyand CM, Goronzy JJ.
J Leukoc Biol. 2008 Sep;84(3):824-34. doi: 10.1189/jlb.0807583. Epub 2008 Jun 27.
18586981

BACKGROUND

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.

REFERENCES

Peterson, E.J., et al., Cancer Res. 63(20):6579-6582 (2003). Leu, Y.W., et al., Cancer Res. 63(19):6110-6115 (2003). Saito, Y., et al., Int. J. Cancer 105(4):527-532 (2003). Siedlecki, P., et al., Biochem. Biophys. Res. Commun. 306(2):558-563 (2003). Macaluso, M., et al., Oncogene 22(23):3511-3517 (2003).

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