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HOXA10 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - HOXA10 Antibody (Center) AP10276C
    Western blot analysis of lysate from T47D cell line, using HOXA10 Antibody (Center)(Cat. #AP10276c). AP10276c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • 3 - HOXA10 Antibody (Center) AP10276C
    Confocal immunofluorescent analysis of HOXA10 Antibody (Center) (Cat#AP10276c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • 1 - HOXA10 Antibody (Center) AP10276C
    HOXA10 Antibody (Center) (Cat. #AP10276c) western blot analysis in WiDr,HepG2 cell line lysates (35ug/lane).This demonstrates the HOXA10 antibody detected the HOXA10 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, E
Primary Accession P31260
Other Accession NP_061824.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42414 Da
Additional info
Gene ID 3206
Other Names Homeobox protein Hox-A10, Homeobox protein Hox-18, Homeobox protein Hox-1H, PL, HOXA10, HOX1H
Target/Specificity This HOXA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-271 amino acids from the Central region of human HOXA10.
Dilution WB~~1:1000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHOXA10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HOXA10
Synonyms HOX1H
Function Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to the DNA sequence 5'-AA[AT]TTTTATTAC-3'.
Cellular Location Nucleus.
Research Areas

BACKGROUND

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor that may regulate gene expression, morphogenesis, and differentiation. More specifically, it may function in fertility, embryo viability, and regulation of hematopoietic lineage commitment. Alternatively spliced transcript variants encoding different isoforms have been described.

REFERENCES

Du, H., et al. Biol. Reprod. 83(2):205-211(2010)
Cheng, W., et al. J. Cancer Res. Clin. Oncol. 136(8):1221-1227(2010)
Liatsikos, S.A., et al. Reprod. Biomed. Online 21(1):126-132(2010)
Godbole, G., et al. J. Reprod. Immunol. 85(2):130-139(2010)
Ko, S.Y., et al. Mol. Cell. Endocrinol. 317 (1-2), 112-119 (2010) :

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