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RPS6 (Ser240/244) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RPS6 (Ser240/244) Antibody AP10275a
    Western blot analysis of lysate from HeLa cell line, using RPS6 Antibody(Ser240/244)(Cat. #AP10275a). AP10275a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
  • 14 - RPS6 (Ser240/244) Antibody AP10275a
    RPS6 antibody (Ser240/244) (Cat. #AP10275a) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS6 antibody (Ser240/244) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - RPS6 (Ser240/244) Antibody AP10275a
    RPS6 Antibody (Ser240/244) (Cat. #AP10275a) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P62753
Other Accession P39017, P62755, P62754, Q4R4K6, P47838, Q5E995, NP_001001.2
Reactivity Human
Predicted Bovine, Chicken, Monkey, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 28681 Da
Additional info
Gene ID 6194
Other Names 40S ribosomal protein S6, Phosphoprotein NP33, RPS6
Target/Specificity This RPS6 (Ser240/244) Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from human RPS6.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPS6 (Ser240/244) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS6
Function May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Research Areas

BACKGROUND

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

REFERENCES

Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008)
Fujita, K., et al. Acta Neuropathol. 116(4):439-445(2008)
Robledo, S., et al. RNA 14(9):1918-1929(2008)
Glover, E.I., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295 (2), R604-R610 (2008) :
Ma, X.M., et al. Cell 133(2):303-313(2008)

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