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PEMT Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PEMT Antibody (N-term) AP1025a
    Western blot analysis of lysates from CEM, rat L6 cell line and human liver tissue lysate(from left to right), using PEMT Antibody (N-term)(Cat. #AP1025a). AP1025a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • 1 - PEMT Antibody (N-term) AP1025a
    Western blot analysis of anti-PEMT Antibody (N-term) Pab (Cat.#AP1025a) in mouse kidney tissue lysates (35ug/lane). PEMT(arrow) was detected using the purified Pab.
  • 14 - PEMT Antibody (N-term) AP1025a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PEMT antibody (N-term) (Cat.#AP1025a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9UBM1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22134 Da
Additional info
Gene ID 10400
Other Names Phosphatidylethanolamine N-methyltransferase, PEAMT, PEMT, PEMT2, PEMT, PEMPT, PNMT
Target/Specificity This PEMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PEMT.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPEMT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PEMT
Synonyms PEMPT, PNMT
Function Catalyzes three sequential methylation reactions of phosphatidylethanolamine (PE) by AdoMet, thereby producing phosphatidylcholine (PC).
Cellular Location Isoform 1: Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Note=Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria.
Research Areas

BACKGROUND

This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Alternate splicing of this gene results in three transcript variants encoding two different isoforms.

REFERENCES

Walkey C.J., Biochim. Biophys. Acta 1436:405-412(1999).
Shields D.J., Biochim. Biophys. Acta 1532:105-114(2001).
Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).

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