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NNMT Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - NNMT Antibody (Center) AP1024C
    All lanes : Anti-NNMT Antibody (Center) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - NNMT Antibody (Center) AP1024C
    Western blot analysis of NNMT (arrow) using rabbit polyclonal NNMT Antibody (Center)(Cat# AP1024c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NNMT gene (Lane 2) (Origene Technologies).
  • 14 - NNMT Antibody (Center) AP1024C
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with NNMT antibody (Center) (Cat# AP1024c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P40261
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 29574 Da
Additional info
Gene ID 4837
Other Names Nicotinamide N-methyltransferase, NNMT
Target/Specificity This NNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the Central region of human NNMT.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNNMT Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NNMT
Function Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
Cellular Location Cytoplasm.
Tissue Location Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas
Research Areas

BACKGROUND

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. NNMT is the protein responsible for this enzymatic activity, which uses S-adenosyl methionine as the methyl donor.

REFERENCES

Xu,J., Thyroid 16 (2), 151-160 (2006)
Roessler,M., Clin. Cancer Res. 11 (18), 6550-6557 (2005)
Souto,J.C., Am. J. Hum. Genet. 76 (6), 925-933 (2005)

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