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Dnmt3A/Dnmt3A2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Dnmt3A/Dnmt3A2 Antibody (C-term) AP1023b
    Western blot analysis of DNMT3A Antibody (C-term) (Cat.# AP1023b) in HepG2 cell line lysates (35ug/lane). DNMT3A (arrow) was detected using the purified Pab.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9Y6K1
Other Accession Q1LZ53, O88508
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 101858 Da
Additional info
Gene ID 1788
Other Names DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A
Target/Specificity This Dnmt3A/Dnmt3A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 723-752 amino acids from the C-terminal region of human Dnmt3A/Dnmt3A2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDnmt3A/Dnmt3A2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNMT3A
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
Cellular Location Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin
Tissue Location Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
Research Areas
Imprinted and DNA methyltransferase gene expression in the endometrium during the pre- and peri-implantation period in cattle.
Author : O'Doherty AM,O'Shea LC,Sandra O,Lonergan P,Fair T,Forde N.
Reprod Fertil Dev. 2016 Sep 21. doi: 10.1071/RD16238. [Epub ahead of print]
27651295
Bovine DNA methylation imprints are established in an oocyte size-specific manner, which are coordinated with the expression of the DNMT3 family proteins.
Author : O\'Doherty AM1, O\'Shea LC, Fair T.
Biol Reprod. 2012 Mar 19;86(3):67. doi: 10.1095/biolreprod.111.094946. Print 2012 Mar.
22088914

BACKGROUND

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. DNMT3A is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

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