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M JMJD3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - M JMJD3 Antibody (C-term) AP1022b
    Western blot analysis of anti-JMJD3 C-term Pab (Cat. #AP1022b) in untransfected 293 cells (left) and transfected 293 cells (right). The detection of a prominent band at 180kDa is observed on transfected 293 cells but not on the untransfected 293 cells. Data kindly provided by Dr.Gioacchino Natoli of European Institute of Oncology.
  • 14 - M JMJD3 Antibody (C-term) AP1022b
    Formalin-fixed and paraffin-embedded human Skeletal Muscle tissue reacted with JMJD3 Antibody(C-term)(Cat.#AP1022b), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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  • 文献引用 : 6
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q5NCY0
Other Accession O15054
Reactivity Mouse
Predicted Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 216850
Other Names Lysine-specific demethylase 6B, 11411-, JmjC domain-containing protein 3, Jumonji domain-containing protein 3, Kdm6b, Jmjd3, Kiaa0346
Target/Specificity This Mouse JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1606-1641 amino acids from the C-terminal region of mouse JMJD3.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsM JMJD3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Kdm6b
Synonyms Jmjd3, Kiaa0346
Function Histone demethylase that specifically demethylates 'Lys- 27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member- dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (PubMed:21095589).
Cellular Location Nucleus.
Research Areas
Jmjd3 mediates blood-spinal cord barrier disruption after spinal cord injury by regulating MMP-3 and MMP-9 expressions.
Author : Lee JY1, Na WH2, Choi HY1, Lee KH2, Ju BG3, Yune TY4.
Neurobiol Dis. 2016 Jul 15;95:66-81. doi: 10.1016/j.nbd.2016.07.015. [Epub ahead of print]
27425890
Opposing roles of STAT4 and Dnmt3a in Th1 gene regulation.
Author : Pham D1, Yu Q, Walline CC, Muthukrishnan R, Blum JS, Kaplan MH.
J Immunol. 2013 Jul 15;191(2):902-11. doi: 10.4049/jimmunol.1203229. Epub 2013 Jun 14.
23772023
Gene networking and inflammatory pathway analysis in a JMJD3 knockdown human monocytic cell line.
Author : Das ND, Jung KH, Choi MR, Yoon HS, Kim SH, Chai YG.
Cell Biochem Funct. 2012 Apr;30(3):224-32. doi: 10.1002/cbf.1839. Epub 2012 Jan 18.
22252741
p53 interaction with JMJD3 results in its nuclear distribution during mouse neural stem cell differentiation.
Author : Solá S, Xavier JM, Santos DM, Aranha MM, Morgado AL, Jepsen K, Rodrigues CM.
PLoS One. 2011 Mar 31;6(3):e18421. doi: 10.1371/journal.pone.0018421.
21483786
Human papillomavirus E7 oncoprotein induces KDM6A and KDM6B histone demethylase expression and causes epigenetic reprogramming.
Author : McLaughlin-Drubin ME1, Crum CP, Münger K.
Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):2130-5. doi: 10.1073/pnas.1009933108. Epub 2011 Jan 18.
21245294
The role of NF-kappaB and H3K27me3 demethylase, Jmjd3, on the anthrax lethal toxin tolerance of RAW 264.7 cells.
Author : Das ND, Jung KH, Chai YG.
PLoS One. 2010 Mar 29;5(3):e9913. doi: 10.1371/journal.pone.0009913.
20360974

BACKGROUND

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

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