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Dnmt2 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Dnmt2 Antibody AP1021b
    Anti-Dnmt2 Antibody (P361) at 1:1000 dilution + mouse cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - Dnmt2 Antibody AP1021b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession O14717
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 1787
Other Names tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog HsaIIP, DNA MTase homolog HsaIIP, MHsaIIP, PuMet, TRDMT1, DNMT2
Target/Specificity This Dnmt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from human Dnmt2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDnmt2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRDMT1
Synonyms DNMT2
Function Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).
Cellular Location Nucleus.
Tissue Location Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes
Research Areas
Presence of 5-methylcytosine in CpNpG trinucleotides in the human genome.
Author : Lee J, Jang SJ, Benoit N, Hoque MO, Califano JA, Trink B, Sidransky D, Mao L, Moon C.
Genomics. 2010 Aug;96(2):67-72. doi: 10.1016/j.ygeno.2010.03.013. Epub 2010 Apr 9.
20382216

BACKGROUND

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a protein with similarity to DNA methyltransferases, but this protein does not display methyltransferase activity. The protein strongly binds DNA, suggesting that it may mark specific sequences in the genome. Alternative splicing results in multiple transcript variants encoding different isoforms.

REFERENCES

Hermann, A., et al., J. Biol. Chem. 278(34):31717-31721 (2003).
Franchina, M., et al., Int. J. Biochem. Cell Biol. 33(11):1104-1115 (2001).
Dong, A., et al., Nucleic Acids Res. 29(2):439-448 (2001).
Yoder, J.A., et al., Hum. Mol. Genet. 7(2):279-284 (1998).
Van den Wyngaert, I., et al., FEBS Lett. 426(2):283-289 (1998).

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