- 文献引用 : 1
|Application ||WB, IHC-P, E|
|Other Names||tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog HsaIIP, DNA MTase homolog HsaIIP, MHsaIIP, PuMet, TRDMT1, DNMT2|
|Target/Specificity||This Dnmt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from human Dnmt2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Dnmt2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).|
|Tissue Location||Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes|
Author : Lee J, Jang SJ, Benoit N, Hoque MO, Califano JA, Trink B, Sidransky D, Mao L, Moon C.
Genomics. 2010 Aug;96(2):67-72. doi: 10.1016/j.ygeno.2010.03.013. Epub 2010 Apr 9.
Provided below are standard protocols that you may find useful for product applications.
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a protein with similarity to DNA methyltransferases, but this protein does not display methyltransferase activity. The protein strongly binds DNA, suggesting that it may mark specific sequences in the genome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Hermann, A., et al., J. Biol. Chem. 278(34):31717-31721 (2003).
Franchina, M., et al., Int. J. Biochem. Cell Biol. 33(11):1104-1115 (2001).
Dong, A., et al., Nucleic Acids Res. 29(2):439-448 (2001).
Yoder, J.A., et al., Hum. Mol. Genet. 7(2):279-284 (1998).
Van den Wyngaert, I., et al., FEBS Lett. 426(2):283-289 (1998).