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MAF1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MAF1 Antibody (Center) AP10204c
    MAF1 Antibody (Center) (Cat. #AP10204c) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the MAF1 antibody detected the MAF1 protein (arrow).
  • 14 - MAF1 Antibody (Center) AP10204c
    MAF1 antibody (Center) (Cat. #AP10204c) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAF1 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - MAF1 Antibody (Center) AP10204c
    MAF1 Antibody (Center) (Cat. #AP10204c) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q9H063
Other Accession Q5XIH0, Q9D0U6, A5D9C6, NP_115648.2
Reactivity Human, Mouse
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 28771 Da
Additional info
Gene ID 84232
Other Names Repressor of RNA polymerase III transcription MAF1 homolog, MAF1
Target/Specificity This MAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-117 amino acids from the Central region of human MAF1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAF1
Function Element of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA.
Cellular Location Nucleus. Cytoplasm.
Research Areas
Maf1 and Repression of RNA Polymerase III-Mediated Transcription Drive Adipocyte Differentiation.
Author : Chen CY1,Lanz RB2,Walkey CJ2,Chang WH3,Lu W4,Johnson DL5.
Cell Rep. 2018 Aug 14;24(7):1852-1864. doi: 10.1016/j.celrep.2018.07.046.
30110641
Covalent small ubiquitin-like modifier (SUMO) modification of Maf1 protein controls RNA polymerase III-dependent transcription repression.
Author : Rohira AD1, Chen CY, Allen JR, Johnson DL.
J Biol Chem. 2013 Jun 28;288(26):19288-95. doi: 10.1074/jbc.M113.473744. Epub 2013 May 14.
23673667

BACKGROUND

This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression.

REFERENCES

Kantidakis, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(26):11823-11828(2010)
Shor, B., et al. J. Biol. Chem. 285(20):15380-15392(2010)
Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Rollins, J., et al. Int. J. Biol. Sci. 3(5):292-302(2007)

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