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MED25 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MED25 Antibody (N-term) AP10203a
    MED25 Antibody (N-term) (Cat. #AP10203a) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MED25 antibody detected the MED25 protein (arrow).
  • 1 - MED25 Antibody (N-term) AP10203a
    MED25 Antibody (N-term) ((Cat. #AP10203a) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MED25 antibody detected the MED25 protein (arrow).
  • 14 - MED25 Antibody (N-term) AP10203a
    MED25 antibody (N-term) (Cat. #AP10203a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MED25 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q71SY5
Other Accession Q8VCB2, A2VE44, NP_112235.2
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 78171 Da
Additional info
Gene ID 81857
Other Names Mediator of RNA polymerase II transcription subunit 25, Activator interaction domain-containing protein 1, Activator-recruited cofactor 92 kDa component, ARC92, Mediator complex subunit 25, p78, MED25, ACID1, ARC92, PTOV2
Target/Specificity This MED25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the N-terminal region of human MED25.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMED25 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MED25
Synonyms ACID1, ARC92, PTOV2
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.
Cellular Location Nucleus.
Tissue Location Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen.
Research Areas
MED25 is a mediator component of HNF4α-driven transcription leading to insulin secretion in pancreatic beta-cells.
Author : Han EH1,Rha GB,Chi YI.
PLoS One. 2012;7(8):e44007. doi: 10.1371/journal.pone.0044007. Epub 2012 Aug 30.
22952853

BACKGROUND

This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq].

REFERENCES

Roupelieva, M., et al. J. Gen. Virol. 91 (PT 5), 1138-1149 (2010) : Leal, A., et al. Neurogenetics 10(4):275-287(2009) Lee, H.K., et al. EMBO J. 26(15):3545-3557(2007) Sato, S., et al. Mol. Cell 14(5):685-691(2004) Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101(8):2339-2344(2004)

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