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ATP5H Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - ATP5H Antibody (Center) AP10185c
    ATP5H antibody (Center) (Cat. #AP10185c) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5H antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - ATP5H Antibody (Center) AP10185c
    ATP5H Antibody (Center) (Cat. #AP10185c) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 1 - ATP5H Antibody (Center) AP10185c
    ATP5H Antibody (Center) (Cat. #AP10185c) western blot analysis in HepG2,NCI-H460,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the ATP5H antibody detected the ATP5H protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession O75947
Other Accession P13620, NP_001003785.1, NP_006347.1
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 18491 Da
Additional info
Gene ID 10476
Other Names ATP synthase subunit d, mitochondrial, ATPase subunit d, ATP5H
Target/Specificity This ATP5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the Central region of human ATP5H.
Dilution IHC-P~~1:50~100
FC~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP5H Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP5PD (HGNC:845)
Synonyms ATP5H
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Cellular Location Mitochondrion. Mitochondrion inner membrane.
Research Areas

BACKGROUND

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.

REFERENCES

Martins-de-Souza, D., et al. J Psychiatr Res 43(11):978-986(2009) Kim, D.W., et al. Cancer Sci. 99(10):1884-1891(2008) Cross, R.L. Nature 427(6973):407-408(2004) Oster, G., et al. Trends Cell Biol. 13(3):114-121(2003) Leyva, J.A., et al. Mol. Membr. Biol. 20(1):27-33(2003)

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