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DNA2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - DNA2 Antibody (Center) AP10182c
    Western blot analysis of lysate from K562 cell line, using DNA2 Antibody (Center)(Cat. #AP10182c). AP10182c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
  • 14 - DNA2 Antibody (Center) AP10182c
    DNA2 antibody (Center) (Cat. #AP10182c) immunohistochemistry analysis in formalin fixed and paraffin embedded human thymus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNA2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - DNA2 Antibody (Center) AP10182c
    DNA2 Antibody (Center) (Cat. #AP10182c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IHC-P, E
Primary Accession P51530
Other Accession D3ZG52, Q6ZQJ5, NP_001073918.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 1763
Other Names DNA replication ATP-dependent helicase/nuclease DNA2, hDNA2, DNA replication ATP-dependent helicase-like homolog, DNA replication nuclease DNA2, 31--, DNA replication ATP-dependent helicase DNA2, DNA2, DNA2L, KIAA0083
Target/Specificity This DNA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 330-358 amino acids from the Central region of human DNA2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDNA2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNA2
Synonyms DNA2L, KIAA0083
Function Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double- strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)- dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.
Cellular Location Nucleus. Mitochondrion. Note=Was initially reported to be exclusively mitochondrial (PubMed:18995831) However, it was later shown to localize both in mitochondrion and nucleus (PubMed:19487465).
Research Areas
hDNA2 nuclease/helicase promotes centromeric DNA replication and genome stability.
Author : Li Z1,Liu B2,Jin W1,3,Wu X4,Zhou M1,Liu VW1,5,Goel A6,Shen Z2,Zheng L7,Shen B7.
EMBO J. 2018 May 17. pii: e96729. doi: 10.15252/embj.201796729. [Epub ahead of print]
29773570
CDK1 phosphorylates WRN at collapsed replication forks.
Author : Palermo V1, Rinalducci S2, Sanchez M3, Grillini F1, Sommers JA4, Brosh RM Jr4, Zolla L2, Franchitto A5, Pichierri P1.
Nat Commun. 2016 Sep 16;7:12880. doi: 10.1038/ncomms12880.
27634057

BACKGROUND

DNA2 is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability (Duxin et al., 2009 [PubMed 19487465]).

REFERENCES

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Kang, Y.H., et al. Crit. Rev. Biochem. Mol. Biol. 45(2):71-96(2010)
Balakrishnan, L., et al. J. Biol. Chem. 285(7):4398-4404(2010)
Duxin, J.P., et al. Mol. Cell. Biol. 29(15):4274-4282(2009)
Zheng, L., et al. Mol. Cell 32(3):325-336(2008)

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