- 文献引用 : 2
|Application ||WB, FC, IHC-P, E|
|Other Accession||D3ZG52, Q6ZQJ5, NP_001073918.1|
|Other Names||DNA replication ATP-dependent helicase/nuclease DNA2, hDNA2, DNA replication ATP-dependent helicase-like homolog, DNA replication nuclease DNA2, 31--, DNA replication ATP-dependent helicase DNA2, DNA2, DNA2L, KIAA0083|
|Target/Specificity||This DNA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 330-358 amino acids from the Central region of human DNA2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DNA2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double- strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)- dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.|
|Cellular Location||Nucleus. Mitochondrion. Note=Was initially reported to be exclusively mitochondrial (PubMed:18995831) However, it was later shown to localize both in mitochondrion and nucleus (PubMed:19487465).|
Author : Li Z1,Liu B2,Jin W1,3,Wu X4,Zhou M1,Liu VW1,5,Goel A6,Shen Z2,Zheng L7,Shen B7.
EMBO J. 2018 May 17. pii: e96729. doi: 10.15252/embj.201796729. [Epub ahead of print]
Author : Palermo V1, Rinalducci S2, Sanchez M3, Grillini F1, Sommers JA4, Brosh RM Jr4, Zolla L2, Franchitto A5, Pichierri P1.
Nat Commun. 2016 Sep 16;7:12880. doi: 10.1038/ncomms12880.
Provided below are standard protocols that you may find useful for product applications.
DNA2 is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability (Duxin et al., 2009 [PubMed 19487465]).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Kang, Y.H., et al. Crit. Rev. Biochem. Mol. Biol. 45(2):71-96(2010)
Balakrishnan, L., et al. J. Biol. Chem. 285(7):4398-4404(2010)
Duxin, J.P., et al. Mol. Cell. Biol. 29(15):4274-4282(2009)
Zheng, L., et al. Mol. Cell 32(3):325-336(2008)