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ACTB Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ACTB Antibody AP10168a
    Western blot analysis of lysates from A431, HepG2 cell line (from left to right), using ACTB Antibody(Cat. #AP10168a). AP10168a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • 14 - ACTB Antibody AP10168a
    ACTB Antibody (Cat. #AP10168a) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - ACTB Antibody AP10168a
    ACTB Antibody (Cat. #AP10168a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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  • 文献引用 : 4
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession P60709
Other Accession A2BDB0, P63259, P63260, P63261, Q5ZMQ2, P63258, P60711, Q6QAQ1, P60710, Q4R561, P60706, P60712, P53505, NP_001092.1
Reactivity Human
Predicted Mouse, Rat, Monkey, Pig, Chicken, Bovine, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 60
Other Names Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
Target/Specificity This ACTB antibody is generated from rabbits immunized with a recombinant protein from human ACTB.
Dilution WB~~1:500-1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACTB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTB
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G- actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947).
Cellular Location Cytoplasm, cytoskeleton Nucleus. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
Research Areas
Cysteine-rich protein 61 regulates adipocyte differentiation from mesenchymal stem cells through mammalian target of rapamycin complex 1 and canonical Wnt signaling.
Author : Yang Y1,2,Qi Q1,2,Wang Y1,2,Shi Y1,2,Yang W1,2,Cen Y1,2,Zhu E1,2,Li X3,Chen D4,Wang B1,2.
FASEB J. 2018 Jan 18:fj201700830RR. doi: 10.1096/fj.201700830RR. [Epub ahead of print]
29401606
Expression of pituitary tumor-transforming 2 in human glioblastoma cell lines and its role in glioblastoma tumorigenesis.
Author : Guo Y1, Shao Y2, Chen J3, Xu S1, Zhang X4, Liu H4.
Exp Ther Med. 2016 May;11(5):1847-1852. Epub 2016 Mar 15.
27168815
Layer 2/3 pyramidal cells in the medial prefrontal cortex moderate stress induced depressive behaviors.
Author : Shrestha P1, Mousa A1, Heintz N1.
Elife. 2015 Sep 15;4. doi: 10.7554/eLife.08752.
26371510
Effect of 5-caffeoylquinic acid on the NF-κB signaling pathway, peroxisome proliferator-activated receptor gamma 2, and macrophage infiltration in high-fat diet-fed Sprague-Dawley rat adipose tissue.
Author : Liu SL1, Peng BJ, Zhong YL, Liu YL, Song Z, Wang Z.
Food Funct. 2015 Jul 9. [Epub ahead of print]
26158223

BACKGROUND

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

REFERENCES

Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010) Ambrosino, C., et al. Mol. Cell Proteomics 9(6):1352-1367(2010) Yoo, Y., et al. Oncogene 29(2):263-272(2010) Boratynska, A., et al. Acta Virol. 54(1):41-48(2010) Dugina, V., et al. J. Cell. Sci. 122 (PT 16), 2980-2988 (2009) :

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