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BACH2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - BACH2 Antibody (C-term) AP10133b
    Overlay histogram showing Ramos cells stained with AP10133b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10133b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - BACH2 Antibody (C-term) AP10133b
    All lanes : Anti-BACH2 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - BACH2 Antibody (C-term) AP10133b
    BACH2 Antibody (C-term) (Cat. #AP10133b) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession Q9BYV9
Other Accession NP_001164265.1, NP_068585.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 60468
Other Names Transcription regulator protein BACH2, BTB and CNC homolog 2, BACH2
Target/Specificity This BACH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 815-841 amino acids from the C-terminal region of human BACH2.
Dilution FC~~1:10~50
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBACH2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BACH2
Function Transcriptional regulator that acts as repressor or activator. Binds to Maf recognition elements (MARE). Play important roles in coordinating transcription activation and repression by MAFK (By similarity). Induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1.
Cellular Location Cytoplasm. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00978, ECO:0000269|PubMed:17018862}. Note=Nucleocytoplasmic shuttling is controlled by phosphorylation
Tissue Location B-cell specific.
Research Areas
A genome-wide regulatory network identifies key transcription factors for memory CD8⁺ T-cell development.
Author : Hu G1, Chen J1.
Nat Commun. 2013;4:2830. doi: 10.1038/ncomms3830.
24335726

REFERENCES

Hinks, A., et al. Ann. Rheum. Dis. (2010) In press :
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
Pierce, B.L., et al. Hum. Hered. 69(3):193-201(2010)
Barrett, J.C., et al. Nat. Genet. 41(6):703-707(2009)

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