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AGTR1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 3 - AGTR1 Antibody (Center) AP10119b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling AGTR1 with AP10119B at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing membrane and cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).
  • 1 - AGTR1 Antibody (Center) AP10119b
    Anti-AGTR1 Antibody (Center) at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - AGTR1 Antibody (Center) AP10119b
    AGTR1 Antibody (Center) (Cat. #AP10119b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the AGTR1 antibody detected the AGTR1 protein (arrow).
  • 14 - AGTR1 Antibody (Center) AP10119b
    AGTR1 Antibody (Center) (Cat. #AP10119b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AGTR1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - AGTR1 Antibody (Center) AP10119b
    AGTR1 Antibody (Center) (Cat. #AP10119b) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, IHC-P, FC, E
Primary Accession P30556
Other Accession P34976, P30555, NP_114438.1, NP_114038.1
Reactivity Human
Predicted Pig, Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 41061 Da
Additional info
Gene ID 185
Other Names Type-1 angiotensin II receptor, AT1AR, AT1BR, Angiotensin II type-1 receptor, AT1, AGTR1, AGTR1A, AGTR1B, AT2R1, AT2R1B
Target/Specificity This AGTR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human AGTR1.
Dilution IF~~1:25
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAGTR1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AGTR1
Synonyms AGTR1A, AGTR1B, AT2R1, AT2R1B
Function Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Liver, lung, adrenal and adrenocortical adenomas
Research Areas

BACKGROUND

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. At least five transcript variants have been described for this gene. Additional variants have been described but their full-length nature has not been determined. The entire coding sequence is contained in the terminal exon and is present in all transcript variants. [provided by RefSeq].

REFERENCES

Xu, M., et al. Atherosclerosis 213(1):191-199(2010)
Niu, W., et al. Hypertens. Res. 33(11):1137-1143(2010)
Procopciuc, L.M., et al. Eur. J. Intern. Med. 21(5):414-418(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Schuur, M., et al. J. Neurol. Neurosurg. Psychiatr. (2010) In press :

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