注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   癌症   >   AMACR Antibody (C-term)   

AMACR Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - AMACR Antibody (C-term) AP10110b
    All lanes : Anti-AMACR Antibody (C-term) at 1:1000 dilution Lane 1: human kidney lysate Lane 2: LNCaP whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - AMACR Antibody (C-term) AP10110b
    AMACR Antibody (C-term) (Cat. #AP10110b) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the AMACR antibody detected the AMACR protein (arrow).
  • 14 - AMACR Antibody (C-term) AP10110b
    AMACR antibody (C-term) (Cat. #AP10110b) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMACR antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - AMACR Antibody (C-term) AP10110b
    AMACR Antibody (C-term) (Cat. #AP10110b) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 3 - AMACR Antibody (C-term) AP10110b
    Confocal immunofluorescent analysis of AMACR Antibody (C-term)(Cat. #AP10110b) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, IF, E
Primary Accession Q9UHK6
Other Accession NP_976316.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42387 Da
Additional info
Gene ID 23600
Other Names Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase, AMACR
Target/Specificity This AMACR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the C-terminal region of human AMACR.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAMACR Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AMACR
Function Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.
Cellular Location Peroxisome. Mitochondrion
Research Areas

BACKGROUND

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described.

REFERENCES

Murray, N.P., et al. Oncol. Rep. 24(3):687-692(2010) Sonwalkar, S.A., et al. Histopathology 56(7):900-907(2010) Lakis, S., et al. World J. Gastroenterol. 16(20):2476-2483(2010) Chen, W., et al. Mol. Biol. Rep. 36(3):423-430(2009) Mubiru, J.N., et al. Gene 327(1):89-98(2004)

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价