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ANGPT2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - ANGPT2 Antibody (C-term) AP10103b
    Overlay histogram showing A549 cells stained with AP10103b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10103b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - ANGPT2 Antibody (C-term) AP10103b
    Anti-ANGPT2 Antibody (C-term) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession O15123
Other Accession NP_001138.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 285
Other Names Angiopoietin-2, ANG-2, ANGPT2
Target/Specificity This ANGPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 404-432 amino acids from the C-terminal region of human ANGPT2.
Dilution FC~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsANGPT2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ANGPT2
Function Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Cellular Location Secreted.
Research Areas
Higher metastatic efficiency of KRas G12V than KRas G13D in a colorectal cancer model.
Author : Alamo P1, Gallardo A2, Di Nicolantonio F3, Pavón MA1, Casanova I1, Trias M4, Mangues MA5, Lopez-Pousa A6, Villaverde A7, Vázquez E7, Bardelli A FASEB J. 2015 Feb;29(2):464-76. doi: 10.1096/fj.14-262303. Epub 2014 Oct 30.
25359494
Subcutaneous preconditioning increases invasion and metastatic dissemination in mouse colorectal cancer models.
Author : Alamo P1, Gallardo A, Pavón MA, Casanova I, Trias M, Mangues MA, Vázquez E, Villaverde A, Mangues R, Céspedes MV.
Dis Model Mech. 2014 Mar;7(3):387-96. doi: 10.1242/dmm.013995. Epub 2014 Jan 30.
24487410

BACKGROUND

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.

REFERENCES

Morrissey, C., et al. Prostate 70(16):1799-1808(2010) Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) : Bento, C.F., et al. Exp. Physiol. 95(9):955-970(2010) Vrbacky, F., et al. Hematology 15(4):210-214(2010) Chen, J., et al. Biochem. Biophys. Res. Commun. 398(2):212-216(2010)

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