- 文献引用 : 2
|Application ||WB, FC, E|
|Other Names||Angiopoietin-2, ANG-2, ANGPT2|
|Target/Specificity||This ANGPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 404-432 amino acids from the C-terminal region of human ANGPT2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ANGPT2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.|
Author : Alamo P1, Gallardo A2, Di Nicolantonio F3, Pavón MA1, Casanova I1, Trias M4, Mangues MA5, Lopez-Pousa A6, Villaverde A7, Vázquez E7, Bardelli A
Author : Alamo P1, Gallardo A, Pavón MA, Casanova I, Trias M, Mangues MA, Vázquez E, Villaverde A, Mangues R, Céspedes MV.
Dis Model Mech. 2014 Mar;7(3):387-96. doi: 10.1242/dmm.013995. Epub 2014 Jan 30.
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.
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