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PRMT2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - PRMT2 Antibody (N-term) AP1002a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 1 - PRMT2 Antibody (N-term) AP1002a
    The anti-PRMT2 Pab (Cat. #AP1002a) is used in Western blot to detect PRMT2 in HL60 cell lysate.
  • 1 - PRMT2 Antibody (N-term) AP1002a
    Western blot analysis of PRMT2 (arrow) using rabbit polyclonal PRMT2 Antibody (A37) (Cat. #AP1002a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRMT2 gene.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P55345
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49042 Da
Additional info
Gene ID 3275
Other Names Protein arginine N-methyltransferase 2, 211-, Histone-arginine N-methyltransferase PRMT2, PRMT2, HMT1, HRMT1L1
Target/Specificity This PRMT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-53 amino acids from the N-terminal region of human PRMT2.
Dilution IHC-P~~1:50~100
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRMT2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRMT2
Synonyms HMT1, HRMT1L1
Function Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1- dependent manner). May be involved in growth regulation.
Cellular Location Isoform 1: Cytoplasm. Nucleus. Note=Translocates from the cytoplasm to the nucleus, after hormone exposure. Excluded from nucleolus Isoform PRMT2Beta: Cytoplasm. Nucleus. Nucleus, nucleolus Isoform PRMT2L2: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic
Tissue Location Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord.
Research Areas

BACKGROUND

Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.

REFERENCES

Qi, C., et al., J. Biol. Chem. 277(32):28624-28630 (2002).
Scott, H.S., et al., Genomics 48(3):330-340 (1998).
Katsanis, N., et al., Mamm. Genome 8(7):526-529 (1997).

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