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PLIN3 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 4 - PLIN3 Antibody AM8697b
    Overlay histogram showing HUVEC cells stained with AM8697b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8697b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - PLIN3 Antibody AM8697b
    All lanes : Anti-PLIN3 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Daudi whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession O60664
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG2a,k
Clone Names 1651CT490.66.85
Calculated MW 47075 Da
Additional info
Gene ID 10226
Other Names Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein, Cargo selection protein TIP47, Mannose-6-phosphate receptor-binding protein 1, Placental protein 17, PP17, PLIN3, M6PRBP1, TIP47
Target/Specificity This PLIN3 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-434 amino acids from human PLIN3.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPLIN3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLIN3
Synonyms M6PRBP1, TIP47
Function Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
Cellular Location Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lipid droplet Note=Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
Research Areas

BACKGROUND

Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.

REFERENCES

Diaz E.,et al.Cell 93:433-443(1998).
Than N.G.,et al.Eur. J. Biochem. 258:752-757(1998).
Than N.G.,et al.Tumor Biol. 20:184-192(1999).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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