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RIPK3 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - RIPK3 Antibody AM8682b
    All lanes : Anti-RIPK3 Antibody at 1:1000 dilution Lane 1: HT-29 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: SW620 whole cell lysate Lane 4: THP-1 whole cell lysate Lane 5: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9Y572
Reactivity Human
Predicted Human
Host Mouse
Clonality monoclonal
Isotype IgG1,κ
Clone Names 2013CT892.86.49
Calculated MW 56887 Da
Additional info
Gene ID 11035
Other Names Receptor-interacting serine/threonine-protein kinase 3, 2.7.11.1, RIP-like protein kinase 3, Receptor-interacting protein 3, RIP-3, RIPK3, RIP3
Target/Specificity This RIPK3 antibody is generated from a mouse immunized with a recombinate protein from the human region of human RIPK3.
Dilution WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRIPK3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RIPK3
Synonyms RIP3
Function Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.
Cellular Location Cytoplasm, cytosol. Cell membrane Mitochondrion
Tissue Location Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers
Research Areas

BACKGROUND

Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.

REFERENCES

Yu P.W.,et al.Curr. Biol. 9:539-542(1999).
Sun X.,et al.J. Biol. Chem. 274:16871-16875(1999).
Yang Y.,et al.Biochem. Biophys. Res. Commun. 332:181-187(2005).
Heilig R.,et al.Nature 421:601-607(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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