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SPI1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - SPI1 Antibody AM8640b
    All lanes : Anti-SPI1 Antibody at 1:500-1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P17947
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1835CT291.10.52
Calculated MW 31083 Da
Additional info
Gene ID 6688
Other Names Transcription factor PU.1, 31 kDa-transforming protein, SPI1
Target/Specificity This SPI1 antibody is generated from a mouse immunized with a recombinant protein of human SPI1.
Dilution WB~~1:500-1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPI1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPI1
Function Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00237, ECO:0000269|PubMed:23166356}
Research Areas

BACKGROUND

Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).

REFERENCES

Ray D.,et al.Oncogene 5:663-668(1990).
Ray D.,et al.Oncogene 5:1611-1612(1990).
Li W.B.,et al.Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
Taylor T.D.,et al.Nature 440:497-500(2006).
Rao S.,et al.J. Biol. Chem. 274:11115-11124(1999).

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