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ABCB4 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - ABCB4 Antibody AM8624b
    Anti-ABCB4 Antibody at 1:4000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 142 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P21439
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1808CT346.21.23
Calculated MW 141523 Da
Additional info
Gene ID 5244
Other Names Multidrug resistance protein 3, 3.6.3.44, ATP-binding cassette sub-family B member 4, P-glycoprotein 3, ABCB4, MDR3, PGY3
Target/Specificity This ABCB4 antibody is generated from a mouse immunized with a recombinant protein of human ABCB4.
Dilution WB~~1:4000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsABCB4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABCB4
Synonyms MDR3, PGY3
Function Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.
Cellular Location Cell membrane; Multi-pass membrane protein.
Research Areas

BACKGROUND

Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.

REFERENCES

van der Bliek A.M.,et al.Gene 71:401-411(1988).
Hillier L.W.,et al.Nature 424:157-164(2003).
Scherer S.W.,et al.Science 300:767-772(2003).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Smit J.J.,et al.Biochim. Biophys. Acta 1261:44-56(1995).

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