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MBD2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 14 - MBD2 Antibody AM8622b
    AM8622b staining MBD2 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - MBD2 Antibody AM8622b
    All lanes : Anti-MBD2 Antibody at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession Q9UBB5
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1812CT625.9.45
Calculated MW 43255 Da
Additional info
Gene ID 8932
Other Names Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2
Target/Specificity This MBD2 antibody is generated from a mouse immunized with a recombinant protein between 10-228 amino acids from human MBD2.
Dilution IHC-P~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMBD2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MBD2
Function Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
Cellular Location Nucleus. Note=Nuclear, in discrete foci Detected at replication foci in late S phase
Tissue Location Highly expressed in brain, heart, kidney, stomach, testis and placenta.

BACKGROUND

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.

REFERENCES

Hendrich B.,et al.Mol. Cell. Biol. 18:6538-6547(1998).
Hendrich B.,et al.Mamm. Genome 10:906-912(1999).
Bhattacharya S.K.,et al.Nature 397:579-583(1999).
Ng H.-H.,et al.Nat. Genet. 23:58-61(1999).
Tatematsu K.,et al.Genes Cells 5:677-688(2000).

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