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ME2 Antibody (C-term)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 14 - ME2 Antibody (C-term) AM8621b
    AM8621b staining ME2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - ME2 Antibody (C-term) AM8621b
    All lanes : Anti-ME2 Antibody (C-term) at 1:4000 dilution Lane 1: HL-60 whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P23368
Reactivity Human, Mouse
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 970CT2.1.1.2
Calculated MW 65444 Da
Additional info
Gene ID 4200
Other Names NAD-dependent malic enzyme, mitochondrial, NAD-ME, 1.1.1.38, Malic enzyme 2, ME2
Target/Specificity This ME2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 522-556 amino acids from the C-terminal region of human ME2.
Dilution IHC-P~~1:25
WB~~1:4000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsME2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ME2
Cellular Location Mitochondrion matrix.
Research Areas

REFERENCES

Loeber G.,et al.J. Biol. Chem. 266:3016-3021(1991).
Yanaihara N.,et al.Genomics 72:169-179(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Nusbaum C.,et al.Nature 437:551-555(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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