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ADK Antibody (N-term)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 14 - ADK Antibody (N-term) AM8619b
    AM8619b staining ADK in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - ADK Antibody (N-term) AM8619b
    All lanes : Anti-ADK Antibody (N-term) at 1:4000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P55263
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1103CT3.4.3
Calculated MW 40545 Da
Additional info
Gene ID 132
Other Names Adenosine kinase, AK, 2.7.1.20, Adenosine 5'-phosphotransferase, ADK
Target/Specificity This ADK antibody is generated from a mouse immunized with a recombinant protein between 1-345 amino acids from human ADK.
Dilution IHC-P~~1:25
WB~~1:4000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADK
Function ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Cellular Location Isoform 1: Nucleus.
Tissue Location Widely expressed. Highest level in placenta, liver, muscle and kidney
Research Areas

BACKGROUND

ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.

REFERENCES

Spychala J.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:1232-1237(1996).
Singh B.,et al.Eur. J. Biochem. 241:564-571(1996).
McNally T.,et al.Biochem. Biophys. Res. Commun. 231:645-650(1997).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).

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