注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   代谢   >   GNMT Antibody (C-term)   

GNMT Antibody (C-term)

Purified Mouse Monoclonal Antibody (Mab)

     
标注"皇冠"的产品,是满足高质量标准的产品。
了解更多...
  • 4 - GNMT Antibody (C-term) AM8617b
    Overlay histogram showing U-2 OS cells stained with AM8617b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8617b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NH174309) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - GNMT Antibody (C-term) AM8617b
    Anti-GNMT Antibody (C-term) at 1:4000 dilution + Human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q14749
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG2a,k
Clone Names 1041CT18.2.3
Calculated MW 32742 Da
Additional info
Gene ID 27232
Other Names Glycine N-methyltransferase, 2.1.1.20, GNMT
Target/Specificity This GNMT antibody is generated from a mouse immunized with a recombinant protein between 1-295 amino acids from the human GNMT.
Dilution FC~~1:25
WB~~1:4000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGNMT Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GNMT
Function Catalyzes the methylation of glycine by using S- adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.
Cellular Location Cytoplasm.
Tissue Location Abundant in liver.
Research Areas

BACKGROUND

Catalyzes the methylation of glycine by using S- adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

REFERENCES

Chen Y.-M.A.,et al.Int. J. Cancer 75:787-793(1998).
Chen Y.-M.A.,et al.Genomics 66:43-47(2000).
Mungall A.J.,et al.Nature 425:805-811(2003).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Ogawa H.,et al.Comp. Biochem. Physiol. 106B:601-611(1993).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价