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PDXK Antibody (N-term)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 14 - PDXK Antibody (N-term) AM8616b
    AM8616b staining PDXK in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - PDXK Antibody (N-term) AM8616b
    All lanes : Anti-PDXK Antibody (N-term) at 1:4000 dilution Lane 1: 293 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: U-251 MG whole cell lysate Lane 4: Human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession O00764
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1007CT2.1.1
Calculated MW 35102 Da
Additional info
Gene ID 8566
Other Names Pyridoxal kinase, 2.7.1.35, Pyridoxine kinase, PDXK, C21orf124, C21orf97, PKH, PNK
Target/Specificity This PDXK antibody is generated from a mouse immunized with a recombinant protein between 1-312 amino acids from human PDXK.
Dilution IHC-P~~1:25
WB~~1:4000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDXK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDXK
Synonyms C21orf124, C21orf97, PKH, PNK
Function Required for synthesis of pyridoxal-5-phosphate from vitamin B6.
Cellular Location Cytoplasm.
Tissue Location Ubiquitous. Isoform 3 is detected in adult testis and spermatozoa
Research Areas

BACKGROUND

Required for synthesis of pyridoxal-5-phosphate from vitamin B6.

REFERENCES

Hanna M.C.,et al.J. Biol. Chem. 272:10756-10760(1997).
Fang X.,et al.Asian J. Androl. 6:83-91(2004).
Hattori M.,et al.Nature 405:311-319(2000).
Lee H.-S.,et al.Mol. Cells 10:452-459(2000).
Daub H.,et al.Mol. Cell 31:438-448(2008).

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