注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   细胞生物学   >   RANBP9 Antibody   

RANBP9 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
标注"皇冠"的产品,是满足高质量标准的产品。
了解更多...
  • 14 - RANBP9 Antibody AM8615b
    AM8615b staining RANBP9 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - RANBP9 Antibody AM8615b
    All lanes : Anti-RANBP9 Antibody at 1:2000-1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: U-2OS whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: CCRF-CEM whole cell lysate Lane 5: MDA-MB-231whole cell lysate Lane 6: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q96S59
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1815CT202.22.8.37
Calculated MW 77847 Da
Additional info
Gene ID 10048
Other Names Ran-binding protein 9, RanBP9, BPM-L, BPM90, Ran-binding protein M, RanBPM, RanBP7, RANBP9, RANBPM
Target/Specificity This RANBP9 antibody is generated from a mouse immunized with a recombinant protein between 1-388 amino acids from the human RANBP9.
Dilution IHC-P~~1:25
WB~~1:2000-1:4000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRANBP9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RANBP9
Synonyms RANBPM
Function May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA (By similarity).
Cellular Location Cytoplasm, cytosol. Nucleus. Note==Predominantly cytoplasmic. A phosphorylated form is associated with the plasma membrane
Tissue Location Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung Within the brain, expressed predominantly by neurons in the gray matter of cortex, the granular layer of cerebellum and the Purkinje cells.
Research Areas

BACKGROUND

May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA (By similarity).

REFERENCES

Nishitani H.,et al.Gene 272:25-33(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mungall A.J.,et al.Nature 425:805-811(2003).
Nakamura M.,et al.J. Cell Biol. 143:1041-1052(1998).
Wang Y.,et al.Biochem. Biophys. Res. Commun. 297:148-153(2002).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价