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AFP Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 14 - AFP Antibody AM8611b
    AM8611b staining AFP in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - AFP Antibody AM8611b
    Anti-AFP Antibody at 1:2000 dilution + human fetal Liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - AFP Antibody AM8611b
    Anti-AFP Antibody at 1:500 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P02771
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG2b,k
Clone Names 1087CT8.3.5
Calculated MW 68678 Da
Additional info
Gene ID 174
Other Names Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP
Target/Specificity This AFP antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 281-609 amino acids from human AFP.
Dilution IHC-P~~1:25
WB~~1:500
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAFP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AFP
Synonyms HPAFP
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Cellular Location Secreted.
Tissue Location Plasma. Synthesized by the fetal liver and yolk sac
Research Areas

BACKGROUND

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

REFERENCES

Morinaga T.,et al.Proc. Natl. Acad. Sci. U.S.A. 80:4604-4608(1983).
Gibbs P.E.M.,et al.Biochemistry 26:1332-1343(1987).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Sakai M.,et al.J. Biol. Chem. 260:5055-5060(1985).
McVey J.H.,et al.Hum. Mol. Genet. 2:379-384(1993).

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